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| Faria,Terezinha de Jesus; Ferreira,Rafael Sottero; Yassumoto,Lidiane; Souza,José Roberto Pinto de; Ishikawa,Noemia Kazue; Barbosa,Aneli de Melo. |
| An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ocimum gratissimum; Antifungal activity; Eugenol; Phytopathogenic fungi. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700002 |
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| Custódio,Dayana Lacerda; Burgo,Rafaela Pinheiro; Moriel,Bárbara; Barbosa,Aneli de Melo; Rezende,Maria Ines; Daniel,Juliana Feijó de Souza; Pinto,Jurandir Pereira; Bianchini,Edmilson; Faria,Terezinha de Jesus. |
| The present study describes the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from the leaves of Pimenta pseudocaryophyllus (1.0% w/w) and Tynanthus micranthus (1.1% w/w). GC and GC/MS analysis demonstrated that eugenol was the only component in the T. micranthus essential oil (99.9%) and the major component in the P. pseudocaryophyllus essential oil (92.59%), which also presented methyleugenol, terpinen-4-ol, o-cymene and (E)-caryophyllene, among others. Both the oils presented antimicrobial activity against bacteria, yeast and filamentous fungi tested.The Bioautography test revealed that eugenol was the bioactive component in both the oils against Cladosporium herbarum. This is the first report about the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pimenta pseudocaryophyllus; Tynanthus micranthus; Essential oil; Antimicrobial activity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600013 |
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| Rezende,Maria Inês; Barbosa,Aneli de Melo; Vasconcelos,Ana Flora Dalberto; Endo,Asae Sakurada. |
| Sugarcane bagasse was used as substrate for xylanase production by means of a strain of Trichoderma harzianum Rifai isolated from decaying Aspidosperma sp. (peroba) wood. The bagasse was washed, dried, milled and wetted with minimal salts medium and the cultures grown at 28 ± 2ºC for 7 days. Two extraction methods were tested for enzyme recovery: (A) Tween 80, 0.1% (v/v), in physiological saline, and (B) 50mM sodium acetate buffer, pH 5.0, under agitation (180rpm) for 15, 30 and 60min. After a single extraction, both extraction methods recovered an average of 15U/ml of xylanase activity, independent on the time of shaking. A second and third extraction recovered 10.4 and 6.6U/ml xylanase, respectively. The effect of volume size for extraction, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trichoderma harzianum; Xylanase; Solid state fermentation; Sugarcane bagasse. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100014 |
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